The funds will be used to support the ongoing Red Cross Red Crescent Movement response in the country, following the invasion of Ukraine by Russia.
The appeal has so far reached one million people in both Ukraine and across Europe, providing financial assistance, food, clothing, water and psychosocial care.
“The conflict in Ukraine is one that has deeply touched us at Pragmatic Play. We have valued colleagues and their families directly affected and we are keen to help them and the wider community in Ukraine in whatever way we can,” Pragmatic Play chief executive Julian Jarvis said.
“Our contribution will help ensure that the incredible work that the Red Cross undertakes can continue, and those caught up in the crisis will be able to receive much needed humanitarian support.”
Gibraltar Red Cross chairman Edward Davies added: “Our work is vital as we look to bring aid to those most in need, and through financial assistance from those such as Pragmatic Play, we can make a difference.
“We are incredibly appreciative of this donation and would like to express our thanks to Pragmatic Play.”
Businesses from across the gambling sector have announced a whole host of initiatives to help those impacted by the invasion of Ukraine.
Last month, Ukraine-based Parimatch Tech announced its support for a project aimed at helping Ukrainian refugees find work. Parimatch also partnered WeHelpUkrainians, a platform that provides Ukrainians with aid resources as part of the Ukraine Hospitals Appeal, and set aside funds to provide resources for those fighting for Ukraine.
In March, “Gaming Industry for Ukraine”, a global gaming industry fundraiser that launched to support people in Ukraine, exceeded its initial £250,000 target, with more than £260,000 having now been raised.